Pranav Vyas - CV

                     Dr. Pranav Ramkrishna Vyas

                                           Reader in Microbiology

Education      : M.Sc Microbiology in 1985

                          Ph.D Microbiology in 1992

                          L.L.B in 2001

Date of Birth : June 11, 1961

Experience    : Teaching  ---  8 Years

                          Research ---   12 Years 

 Technical Expertise :

  1. Genetic transformation of Streptomyces and E.coli and recombinant DNA techniques. These include protoplastation , regeneration, isolation of chromosomal and plasmid DNA, RNA isolation, RE digestiuon, southern and northern hybridisation.

  2. Techniques involved in enzyme purification and characterization. These include gel filtration, ion exchange chromatography, preparative and analytical PAGE, isoelectric focussing, SDS-PAGE, western blotting and HPLC.

  3. Immunological techniques such as maintenance  of cell lines, neutrophil isolation from whole blood, subcelluler fractionation  of PMN and cell staining for immunofluorescence microscopy.

  4. Transient transfection of various cell lines such as RAW 264.7 and Chondrosarcoma cells using DEAE-Dextran, Calcium phosphate and Lipofectin  methods.

Awards :

  1. 1999-2000, received Best Poster Award at 41st Annual Conference of Association of

  2. Microbiologists of India, Jaipur, India.

  3. 1989-1991, received Council of Scientific and Industrial Research Senior Research Fellowship, India.

  4. 1986-1988, received Council of Scientific and Industrial Research Junior Research Fellowship, India.

Publications (Last 10 years) :

  1. Vipul Gohel, Vyas P.R., Chhatpar H.S. Activity statining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis. African J. Biotechnol. 4(17) : 87-90 (2005).

  2. Vipul Gohel, Derick Jiwan, Vyas P.R., Chhatpar H.S. Statistical optimization of chitinase production by Pantoea dispersa to enhance degradation of crustacean chitin waste. Journal of Microbiology & Biotechnology, 15(5) : 197-201 (2005).

  3. Vipul Gohel, Derick Jiwan, Smita Tandel, Pranav Vyas, Chhatpar H.S. (2005) Management of the chitinous wastes by production of chitosan and its application.  Environmental Degradation and Management, Vol. 1 35-42, ISBN-81-85543-0504.

  4. Vipul Gohel, Megha C., Vyas P.R., Chhatpar H.S. Strain improvement of chitinase producing isolate Pantoea dispersa for enhancing its biocontrol potential against fungal plant pathogens’ Annals of Microbiology, 54 : 503 (2004).

  5. Vipul Gohel, Smita Trivedi, Vyas P.R., Chhatpar H.S. Formulation of medium constituents by multiresponse analysis of central composite design to enhance chitinase production in Pantoea dispersa. Ind. J. Expt. Biol. 42 : 1123 (2004).

  6. Vipul Gohel, Tejas Chaudhary, Vyas P.R., Chhatpar H.S. Isolation and identification of marine chitinolytic bacteria and their potential in antifungal biocontrol. Ind. J. Expt. Biol. 42 : 715 (2004).

  7. Vaidya RJ, Macmil SLA, Vyas P.R. and Chhatpar HS. The novel method for isolating chitinolytic bacteria and its application in screening for hyperchitinase producing mutant of Alcaligenes xylosoxydansLetters in Applied Microbiology 36: 129-134 (2003).

  8. Vaidya R, Vyas P.R. and Chhatpar HS. Statistical optimization of medium components for the production of chitinase by Alcaligenes xylosoxydans. Enzyme and Microbial Technology 33: 92-96 (2003).
  9. Vaidya RJ, Macmil SLA, Vyas P.R, Ghetiya L, Thakor KJ and Chhatpar HS.  Biological control of Fusarium wilt of pigeonpea Cajanus cajan (L.) Millsp. with chitinolytic Alcaligenes xylosoxydans. Indian Journal of Experimental Biology 41: 1463-1472 (2003).

  10. Vaidya R, Roy S, Macmil S, Gandhi S, Vyas P.R. and Chhatpar HS. Purification and characterization of chitinase from Alcaligenes xylosoxydans. Biotechnology Letters 25: 715-717 (2002).

  11. Vaidya RJ, Shah IM, Vyas P.R. and Chhatpar H.S. Production of chitinase and its optimization from a novel isolate Alcaligenes xylosoxydans: potential in antifungal biocontrol. World Journal of Microbiology and Biotechnology 17 : 691-696 (2001).

  12. Vaidya RJ, Shah IM, Chhaya UD, Vyas P.R. and Chhatpar HS. Chitinase biopesticides for management of fungal plant pathogen. In : Proceedings of International Conference on Managing Natural Resources for Sustainable Agricultural Production in 21st Century, held at New Delhi (2000).

  13. Vaishnav P., Randev S., Jatian S., Aggrawal S., Keharia H., Vyas P.R., Nareshkumar D. and G. Archana. Characterization of carbamoyl phosphate synthetase of Streptomyces spp. s. 38 : 931-935 (2000).

  14. Attur M.G., Patel R., Thakker G., Vyas P.R., Levartovsky D., Patel P., Naquvi S., Raza R., Patel K., Abramson D., Bruno G., Abramson S.B. and Amin A.R. Differential anti-inflammatory effects of immunosuppresive drugs: Cyclosporin, rapamycin and FK-506 on inducible nitirc oxide synthase, nitric oxide cyclooxygenase-2 and PGE2 production. Inflammation Research, 48 : 1-7 (1999)

  15. Patel I.R., Vyas P.R., Attur M., Thakker G., Abramson S.B. and Amin A.R. Cloning and expression of a functional, dimeric, multi-cofactor requiring murine macrophage inducible nitric  oxide  synthase  in  bacteria  to  bacmid  transposition  Baculovirus  system. Transgenics2 : 161-166 (1997).

  16. Amin A.R., Attur M., Thakker G., Patel P.B., Vyas P.R., Patel R.N., Patel I.R. and Abramson S.B. A novel mechanism of action of tetracyclines : Effects on nitric oxide synthases. Proc. Natl. Acad. Sci. USA, 93 : 14014-14019 (1996).

  17. Vyas P.R., Attur M., Ou G.M., Haines K.A.,  Abramson S.B. and Amin A.R. Thin layer chromatography : An effective method to monitor citrulline synthesis by nitric oxide synthase activity. In : Moncada S., Stamler J., Gross S. and Higgs E.A. (eds.) "The Biology of Nitric Oxide", Part 5, London, Portland Press, p. 44 (1996).

  18. Amin A.R., Attur M., Vyas P.R., Joanna L.P., Levartovsky D., Rediske J., Clancy R.M., Vora K. and Abramson S.B. Expression of nitric oxide synthase in human peripheral blood mononuclear cells and neutrophils. J. Inflammation, 47 : 190-205 (1996).

  19. Amin A.R., DiCesare P., Vyas P.R., Attur M., Edith T., Billar T.R. and Abramson S.B. Expression and regulation of nitric oxide synthase in human osteoarthritis affected chondrocytes : Evidence for an inducible "neuronal-like" nitric oxide synthase. J. Exp. Med. 182 : 2097-2102 (1995).

  20. Amin A.R., Vyas P.R., Attur M., Leszczynska-Pizik J., Patel I.R., Weissman G. and Abramson S.B. The mode of action of aspirin like drugs : Effect on inducible nitric oxide synthase. Proc. Natl. Acad. Sci. 92 : 7926-7930 (1995).

  21. Deshpande M.V., Ganapathy S., Vyas P.R., Ravikumar A. and Rajamohan P.R. Solid state CP/MAS carbon 13 NMR spectroscopy : A sensitive method to monitor enzymatic hydrolysis of chitin. J. of Biochemical & Biophysical Methods. 31 : 151-163 (1996).

  22. Deshpande M.V., Ganapathy S., Vyas P.R., Ravikumar A. and Rajamohan P.R. Solid state CP/MAS carbon 13 NMR spectroscopy : A sensitive method to monitor enzymatic hydrolysis of chitin. In Chitin Enzymology. Ed. R.A.A.Muzzarelli, Pub. Eur. Chitin Soc. Ancona, pg.469-476 (1993).

  23. Vyas P.R. and Deshpande M.V. Purification and characterization of an endochitinase from Myrrothecium verrucaria. J. Gen. Appl. Microbiol. 39 : 91-99 (1993).

Membership :

Life Member, Society of Biological Chemists (India)

Conferences/Workshops/Talks attended :

  1.  Participated in a UGC sponsored refresher course in Microbiology organised by Bharatidasan University, Tiruchirapalli, Tamil Nadu during 1st to 21st November 2004.

  2.  Participated in a UGC sponsored refresher course in Molecular microbiology and genetic engineering held at Dept. of Microbiology & Biotechnology Centre, M.S.University of Baroda during 25th September to 15th October 2003.

  3. Presented a paper on ‘Self-examination is true reformation’ in UGC Golden Jubilee Seminar-2002:2003 on examination reforms organised by M.S.University of Baroda, during 11th -13th September 2003.

  4. Presented a paper entitled ‘Science and colonical reasoning in a national workshop on “Philosophical foundations of the discourses of science” organised by the forum on contemporary theory of the M.S.University of Baroda during 29th to 31st October 2002.

  5. Participated UGC-sponsored refresher course in Biosciences organised by Academic Staff College, Ahmedabad during 2nd to 20th November 2000.

  6. Attended annual meeting of American Society of Human Genetics (ASHG) held at San Francisco, USA from 23rd - 28th October 2000.

  7. Attended the fifth annual vision science conference held at Lake Arrowhead, California from 23rd - 25th September 2000.

  8. Attended FASEB meeting in Anaheim, USA, 23rd -28th April 1994.

  9. Mahabaleshwar seminar on "Modern biology; Genetic recombination", held at Green Hill   Campus, Mahabaleshwar, INDIA, 19th – 26th November 1989.

  10. DBT short term training cource on "Genetic manipulation in Streptomyces", held at Department  of Biotechnology, School of Biological Sciences, Madurai Kamraj University, Madurai, INDIA, 1st – 21st November 1987.

  11. UGC short term training programme "Purification and characterization of mRNA in eucaryotes", held at Department of  Zoology, University of Poona, Pune, INDIA, 14th – 28th  March 1987.

Research Projects :

Sr.

No.

Name of the Project

Funding Agency

Period

Status

1)

Studies on the physiology & biochemistry of Deinococcus spp. isolated from varied habitats         (Co-Investigator)

Board of Research in Nuclear Science (BRNS)

2002-2005

Ongoing

2)

Crustacean chitin waste management for the production of chitin, chitosan & biocontrol agents for plant pathogens    (Co-Investigator)

Dept. of Ocean Development,   New Delhi

2002-2005

Ongoing

3)

Isolation of hyper-chitinase producing mutants of M. verrucaria NCIM903 Pseudomonas fluorescens

M.S.University Baroda

2001-2002

Ongoing

4)

Microbiological study of vermicompost

M.S.University Baroda

1999-2001

Completed

5)

Studies for ecological restoration & planning of Alang ship-breaking yard (Co-investigator)

Gujarat Ecological Commission, Baroda

1996-1997

Completed


Copyright © 2002
Bioinformatics Centre , Dept. of Microbiology and Biotechnology Centre
M.S. University of Baroda, Baroda, India 390002 Phone:91-0265-2794396 , Fax: 91-265-2792508

Last updated: Thursday, 17 January, 2008 04:02 PM